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Effective enhancement of polylactic acid-degrading enzyme production by Amycolatopsis sp. strain SCM_MK2-4 using statistical and one-factor-at-a-time approaches.

Authors
  • Penkhrue, Watsana1
  • Kanpiengjai, Apinun2, 3
  • Khanongnuch, Chartchai2
  • Masaki, Kazuo4
  • Pathom-Aree, Wasu1
  • Punyodom, Winita3
  • Lumyong, Saisamorn1
  • 1 a Department of Biology , Faculty of Science, Chiang Mai University, Muang District , Chiang Mai , Thailand. , (Thailand)
  • 2 b Division of Biotechnology, School of Agro-Industry, Faculty of Agro-Industry, Chiang Mai University, Muang District , Chiang Mai , Thailand. , (Thailand)
  • 3 d Department of Chemistry , Faculty of Science, Chiang Mai University, Muang District , Chiang Mai , Thailand. , (Thailand)
  • 4 c National Research Institute of Brewing, Higashihiroshima , Hiroshima , Japan. , (Japan)
Type
Published Article
Journal
Preparative Biochemistry & Biotechnology
Publisher
Informa UK (Taylor & Francis)
Publication Date
Aug 09, 2017
Volume
47
Issue
7
Pages
730–738
Identifiers
DOI: 10.1080/10826068.2017.1315597
PMID: 28414263
Source
Medline
Keywords
License
Unknown

Abstract

This study aims to find the optimal medium and conditions for polylactic acid (PLA)-degrading enzyme production by Amycolatopsis sp. SCM_MK2-4. Screening of the most effective components in the enzyme production medium by Plackett-Burman design revealed that the silk cocoon and PLA film were the most significant variables enhancing the PLA-degrading enzyme production. After an response surface methodology, a maximum amount of PLA-degrading enzyme activity at 0.74 U mL-1 was predicted and successfully validated at 95% after 0.39% (w/v) silk cocoon and 1.62% (w/v) PLA film were applied to the basal medium. The optimal initial pH value, temperature, and inoculum size were evaluated by a method considering one-factor-at-a-time. The values were recorded at an initial pH in the range of 7.5-9.0, a temperature of 30-32°C, and an inoculum size of 4-10%. The highest activity of approximately 0.95 U mL-1 was achieved after 4 days of cultivation using the optimized medium and under optimized conditions in a shake flask. Upscaling to the use of a 3-L stirred tank fermenter was found to be successful with a PLA-degrading activity of 5.53 U mL-1; which represents a 51-fold increase in the activity compared with that obtained from the nonoptimized medium and conditions in the shake flask.

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