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Effect of in vivo L-acetylcarnitine administration on ATP-ases enzyme systems of synaptic plasma membranes from rat cerebral cortex.

Authors
  • Villa, Roberto Federico
  • Ferrari, Federica
  • Gorini, Antonella
Type
Published Article
Journal
Neurochemical Research
Publisher
Springer-Verlag
Publication Date
Aug 01, 2011
Volume
36
Issue
8
Pages
1372–1382
Identifiers
DOI: 10.1007/s11064-011-0462-x
PMID: 21479591
Source
Medline
License
Unknown

Abstract

The maximum rates (V (max)) of some enzymatic activities related to energy consumption (ATP-ases) were evaluated in two types of synaptic plasma membranes (SPM) isolated from cerebral cortex of rats subjected to in vivo treatment with L: -acetylcarnitine at two different doses (30 and 60 mg kg(-1) i.p., 28 days, 5 days/week). The following enzyme activities were evaluated: acetylcholinesterase (AChE); Na(+), K(+), Mg(2+)-ATP-ase; ouabain insensitive Mg(2+)-ATP-ase; Na(+), K(+)-ATP-ase; direct Mg(2+)-ATP-ase; Ca(2+), Mg(2+)-ATP-ase; Low- and High-affinity Ca(2+)-ATP-ase. Sub-chronic treatment with L: -acetylcarnitine increased Na(+), K(+)-ATP-ase activity on SPM 2 and Ca(2+), Mg(2+)-ATP-ase activity on both SPM fractions. These results suggest (1) that the sensitivity to drug treatment is different between the two populations of SPM, confirming the micro-heterogeneity of these sub-fractions, probably originating from different types of synapses, (2) the specificity of the molecular site of action of the drug on SPM and (3) its interference on ion homeostasis at synaptic level.

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