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Effect of Transglutaminase on the Properties of Films Prepared from Chitosan and Gelatin

Authors
  • Kadimaliev, D. A.1
  • Parchaykina, O. V.1
  • Syusin, I. V.1
  • Chairkin, I. V.2
  • Malafeev, A. N.1
  • Devyatkin, A. A.1
  • Revin, V. V.1
  • 1 Ogarev Mordovia State National Research University, Faculty of Biotechnology and Biology, Saransk, 430005, Russia , Saransk (Russia)
  • 2 Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, 119991, Russia , Moscow (Russia)
Type
Published Article
Journal
Applied Biochemistry and Microbiology
Publisher
Pleiades Publishing
Publication Date
May 01, 2021
Volume
57
Issue
3
Pages
366–372
Identifiers
DOI: 10.1134/S0003683821030042
Source
Springer Nature
Keywords
License
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Abstract

AbstractThe production conditions were optimized, and the characteristics of biodegradable chitosan-gelatin films crosslinked with various microbial transglutaminases (transglutaminase 1, which contains maltodextrin, and transglutaminase 2, which contains maltodextrin, a milk protein) were studied. The film properties depended on both the cross-linking conditions and the type and ratio of components in the mixture. Gels and films prepared from chitosan and gelatin with transglutaminase 1 had very low characteristics as compared to films obtained with transglutaminase 2. Infrared spectroscopy, X-ray tomography, and scanning atomic force electron microscopy showed that transglutaminase 2 forms cross-links between both gelatin and chitosan amino groups, which improves the film characteristics. Films prepared from chitosan, gelatin, and glycerol crosslinked with transglutaminase 2 had the following characteristics: a strength of 15.8 MPa, a tensile strength of 220.0%, a thickness of 73.1 μm, and a density of 1.9 g/cm3. The biodegradability of the films depended on the composition and ranged from 20 to 30 days. The resulting films can serve as a matrix to obtain medical materials with antibacterial and wound-healing properties.

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