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Effect of transforming growth factor-β on lipoprotein lipase in rat mesenchymal heart cell cultures

Authors
  • Friedman, G.
  • Ben-Yehuda, A.
  • Ben-Naim, M.
  • Matsa, D.
  • Stein, O.
  • Stein, Y.
Type
Published Article
Journal
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
Publisher
Elsevier
Publication Date
Jan 01, 1995
Volume
1254
Issue
2
Pages
140–146
Identifiers
DOI: 10.1016/0005-2760(94)00175-X
Source
Elsevier
Keywords
License
Unknown

Abstract

The effect of recombinant transforming growth factor-β2 (rTGF-β2) on lipoprotein lipase (LPL) synthesis was studied in mesenchymal rat heart cell cultures. Addition of rTGF-β2 to culture medium containing 20% serum resulted in a time-dependent decrease in LPL activity. With 10 ng/ml a 30% fall occurred after 12 h and only 20% of enzyme activity remained after 24 h with 5 or 10 ng/ml. The minimal effective dose of rTGF-β2 was 0.1 ng/ml and a 20% decrease occurred after exposure for 24 h. Antibodies specific to TGF-β2 blocked this effect. The decrease in enzymic activity was accompanied by a decrease in enzume mass and LPL mRNA. Addition of rTGF-β2 was effective only during the first week in culture, when enzyme activity was increasing but not after 12 days when the cultures were overconfluent, and the enzyme activity was high.

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