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Effect of temperature on plasmid stability and expression of cloned cellulase gene in a recombinantBacillus megaterium.

Authors
  • Son, Kwang H1
  • Jang, Jong H1
  • Kim, Jung H1
  • 1 Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Chongryang, POB 150, 131, Seoul, Korea. , (North Korea)
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Dec 01, 1987
Volume
9
Issue
12
Pages
821–824
Identifiers
DOI: 10.1007/BF01026189
PMID: 28247147
Source
Medline
Language
English
License
Unknown

Abstract

The expression of carboxymethyl cellulase gene inBacillus megaterium (pCK108) was investigated with respect to temperature in batch culture. The suboptimal temperature supporting maximal cell growth rate was determined to be 30 °C at which stability of the plasmid pCK108 could be maintained stable. However, cellular plasmid contents, production rate of cellulase of the cell, and efficiency of the gene expression increased significantly with increase of the temperature from 30 °C to 44 °C, even though the plasmid stability decreased up to 60% level at the end of the culture.

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