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Effect of supplementation of recombinant Regucalcin in extender on cryopreservation of spermatozoa of water buffalo (Bubalus bubalis).

Authors
  • Pillai, Harikrishna1
  • Parmar, Mehtab S2
  • Shende, Ashish M1
  • Thomas, Jobin3
  • Sharma, Harikumar S4
  • Sharma G, Taru2
  • Ghosh, Subrata K5
  • Kumaresan, Arumugam6
  • Bhure, Sanjeev K1
  • 1 Division of Biochemistry, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India. , (India)
  • 2 Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India. , (India)
  • 3 Immunology Section, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India. , (India)
  • 4 Division of Pharmacology and Toxicology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India. , (India)
  • 5 Germ Plasm Center, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India. , (India)
  • 6 Animal Reproduction, Gynaecology and Obstetrics, ICAR-National Dairy Research Institute, Karnal, Haryana.
Type
Published Article
Journal
Molecular Reproduction and Development
Publisher
Wiley (John Wiley & Sons)
Publication Date
Nov 01, 2017
Volume
84
Issue
11
Pages
1133–1139
Identifiers
DOI: 10.1002/mrd.22873
PMID: 28782859
Source
Medline
Keywords
License
Unknown

Abstract

Elevated intracellular calcium concentration and oxidative damage are two major factors contributing to the poor fertility of cryopreserved spermatozoa. Regucalcin (RGN), also known as Senescence marker protein-30 (SMP-30), is a calcium-binding protein with multiple roles that include calcium homeostasis, anti-oxidative, anti-apoptosis, and anti-proliferation. In Drosophila, RGN is reportedly a putative cold-tolerance gene and a cytoprotective role for RGN against intracellular calcium elevation and oxidative stress was reported in P19 cell lines. Given that RGN has anticapacitatory effect and abundant in the male reproductive tract, we hypothesized that it may play a cryoprotective role for spermatozoa. We investigated this by including RGN, at three different concentrations (20, 40, and 60 μg/ml), as a supplement for Tris-egg yolk-based semen extender. Post-thaw metrics of progressive motility, acrosome integrity, and zona pellucida binding of spermatozoa were evaluated for three ejaculates of three clinically normal, breeding Murrah buffaloes. A concentration of 40 μg/ml of recombinant RGN supplemented during sperm freezing resulted in significant increases in the post-thaw progressive motility of spermatozoa (50.6 ± 3.5% vs 40.6 ± 2.6%; p < 0.01), acrosome integrity (53.3 ± 7.4 vs 75.6 ± 6.8; p < 0.05), and zona pellucida binding (31.6 ± 14.0 vs 191.9 ± 12.3 bound spermatozoa; p < 0.01) compared to control conditions without RGN. Thus, ∼1 μM recombinant RGN, which retains the ability to bind calcium, has a cryoprotective effect for buffalo spermatozoa in extender.

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