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Effect of sperm dosage transportation in stallions: Effect on sperm DNA fragmentation.

Authors
  • de la Torre, J1
  • Crespo, F2
  • Arroyo, F3
  • Zabal-Aguirre, M4
  • Abdoon, A S5
  • Gosálvez, J6
  • 1 Departamento de Biología, Comisión de Genética, Universidad Autónoma de Madrid (UAM), C. Darwin 2, E-28049 Madrid, Spain; Centro de Investigación en Biodiversidad y Cambio Global (CIBC-UAM), Universidad Autónoma de Madrid, C. Darwin 2, E-28049 Madrid, Spain. Electronic address: [email protected] , (Spain)
  • 2 Centro Militar de Cría Caballar de Ávila (FESCCR- Ministerio de Defensa), 05005 Ávila, Spain. , (Spain)
  • 3 Departamento de Biología, Comisión de Genética, Universidad Autónoma de Madrid (UAM), C. Darwin 2, E-28049 Madrid, Spain. , (Spain)
  • 4 Centro de Investigación sobre la Desertificación, CIDE-CSIC, Valencia, Spain. , (Spain)
  • 5 Department of Animal Reproduction & Artificial Insemination, Veterinary Research Division, National Research Center, 12622 Giza, Egypt. , (Egypt)
  • 6 Departamento de Biología, Comisión de Genética, Universidad Autónoma de Madrid (UAM), C. Darwin 2, E-28049 Madrid, Spain; Centro de Investigación en Biodiversidad y Cambio Global (CIBC-UAM), Universidad Autónoma de Madrid, C. Darwin 2, E-28049 Madrid, Spain. , (Spain)
Type
Published Article
Journal
Animal reproduction science
Publication Date
Jul 01, 2019
Volume
206
Pages
38–45
Identifiers
DOI: 10.1016/j.anireprosci.2019.05.005
PMID: 31109754
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Artificial insemination programs for horses usually involve ex vivo handling and transporting of sperm. The present experiment was designed to: (i) assess the effect of transportation on sperm DNA integrity at different time post semen collection, and (ii) evaluate if sperm DNA quality deteriorates rapidly beyond 24 h of cooled storage. After collection, the ejaculates were extended using INRA 96 and semen was prepared for prompt analysis (A0) or 24 h/48 h cooled-shipping (B24 and C48 respectively). Each sample was assessed for sperm DNA fragmentation index (SDFI) at time 0 and after incubation for 2, 6 and 24 h at 37 °C. There was very little difference in SDFI between freshly extended (A0) and 24 h/48 h cooled-transported semen samples (B24/C48) at time 0. After 2 h of incubation at 37 °C, there was an increase in SDFI ranging from 2.7% to 7.5% per hour in freshly extended semen samples (A0: 5.1 ± 1.5), while cooled-transported semen samples had a much greater increase in SDFI, ranging from 5.0% to 20.5% (B24: 14.7 ± 5.6) and from 8.2% to 26.8% (C48: 18.3 ± 7.2) respectively. There were not marked differences in the sperm DNA integrity between 24 and 48 h for transported samples, thus there is the possibility of desirable fertility with use of stallion sperm after 48 h of cooled storage. Copyright © 2019 Elsevier B.V. All rights reserved.

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