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Effect of soya bean and fish oil inclusion in diets on milk and plasma enzymes from sheep and goat related to oxidation.

Authors
  • Tsiplakou, E1
  • Chatzikonstantinou, M2
  • Mitsiopoulou, C1
  • Karaiskou, C1
  • Mavrommatis, A1
  • Sotirakoglou, K3
  • Labrou, N2
  • Zervas, G1
  • 1 Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Athens, Greece. , (Greece)
  • 2 Laboratory of Enzyme Technology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, Athens, Greece. , (Greece)
  • 3 Department of Plant Breeding and Biometry, Agricultural University of Athens, Athens, Greece. , (Greece)
Type
Published Article
Journal
Journal of animal physiology and animal nutrition
Publication Date
Aug 01, 2017
Volume
101
Issue
4
Pages
733–742
Identifiers
DOI: 10.1111/jpn.12516
PMID: 27040180
Source
Medline
Keywords
License
Unknown

Abstract

This study investigated the effects of dietary inclusion of soya bean oil combined with fish oil (SFO) on the activities of a) superoxide dismutase (SOD), glutathione reductase (GR), catalase (CAT) and glutathione transferase (GST) in blood plasma and b) SOD, GR, CAT and lactoperoxidase (LPO) in the milk of sheep and goats. Furthermore, the oxidative stress indicators for measuring total antioxidant activity and free radical scavenging activity [ferric reducing ability of plasma (FRAP) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays] and oxidative stress biomarkers [malondialdehyde (MDA) and protein carbonyl (PC)] were also determined in the blood plasma and milk of the animals. For this purpose, twelve dairy sheep and twelve dairy goats were assigned each to two homogenous subgroups. Treatments in both animal species involved a control diet without added oil and a diet supplemented with 5% soya bean oil and 1% fish oil. The results showed that the inclusion of SFO in the diets of sheep and goats increased significantly the activities of CAT and GR in their blood plasma. The same effect was observed for the activities of GST and FRAP in the blood plasma of goats. Moreover, the fact that the goats had significantly higher average daily PUFA intake (3.62 g/kg BW0.75 ) compared to sheep (2.51 g/kg BW0.75 ) resulted in an enhancement in the MDA content in their plasma. A significant increase in CAT activity in the milk in both animal species fed with SFO diets was also found. Finally, due to the higher apparent transfer rate of n-3 FA from the diet to the milk in sheep, the PC concentrations were found to be enhanced in their plasma and milk. In conclusion, the impact of dietary SFO supplementation on the oxidative status of body and/or on the milk of small ruminants depends not only on the daily PUFA intake, but also on the amount of n-3 FA that reach their milk.

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