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Effect of SERCA pump inhibitors on chemoresponses in Paramecium.

Authors
  • Wassenberg, J J
  • Clark, K D
  • Nelson, D L
Type
Published Article
Journal
The Journal of eukaryotic microbiology
Publication Date
Jan 01, 1997
Volume
44
Issue
6
Pages
574–581
Identifiers
PMID: 9435128
Source
Medline
License
Unknown

Abstract

Inhibitors of SERCA (sarcoplasmic/endoplasmic reticulum Ca(2+)-dependent ATPase) calcium pumps were used to investigate the involvement of internal Ca2+ stores in the GTP response in Paramecium. External application of these inhibitors was found to dramatically alter the typical behavioral and electrophysiological responses of Paramecium to extracellular chemical stimulation. In particular, 2,5-di-tert-butylhydroquinone (BHQ) strongly inhibited the backward swimming response of paramecia to externally applied GTP, though it did not inhibit the associated whirling response. BHQ also prolonged the normally brief electro-physiological response of these cells to GTP. BHQ completely blocked the behavioral and electrophysiological responses of Paramecium to extracellular Ba2+, but had no measurable effect on the behavioral or electrophysiological responses of these cells to another depolarizing stimulus, elevated external K+ concentration. These results suggest the involvement of nonciliary Ca2+ ions in the GTP and Ba2+ responses.

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