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The effect of protease inhibitors, leupeptin and E64d, on differentiation of C2C12 myoblasts in tissue culture.

Authors
  • Kumar, A
  • Shafiq, S
  • Wadgaonkar, R
  • Stracher, A
Type
Published Article
Journal
Cellular and molecular biology
Publication Date
Aug 01, 1992
Volume
38
Issue
5
Pages
477–483
Identifiers
PMID: 1468108
Source
Medline
License
Unknown

Abstract

Intracellular calcium levels play an important role in myofibril disintegration and regeneration of muscle fibers. Earlier studies have shown that the calcium activated protease, calpain, is involved in the removal of Z-discs from myofibrils of striated muscle and the tripeptide-aldehyde, leupeptin, which is an inhibitor of calpain, inhibits this activity. In the present communication, we demonstrate that leupeptin and another calpain inhibitor, E64d, inhibit the fusion of mouse skeletal muscle C2C12 myoblasts to form multinucleated myotubes in tissue culture.

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