This study was aimed to investigate the electrokinetic removal of environmental hormone––nonylphenol (NP)––from soil matrix under potential gradient of 1 V cm−1 for 5-day treatment. The EK experiments were conducted with four processing fluids of de-ionized water, citric acid, NaOH, and methanol in a Pyrex glass cylindrical cell. Results showed that the elcetrokinetic removal efficiency of the above-mentioned processing fluid was 29, 36–38, 43–53, and 53–69%, respectively. It was found that the removal of NP in EK system was highly related to the solubility of NP in processing fluid. Approximate 88.8–94.2% of NP removal was collected in the cathode reservoir after EK treatment, which was 7.9–16.2 times greater than that collected in the anode reservoir. It was concluded that NP was mainly removed from anode to cathode by electroosmosis flow. The electrokinetic phenomenon of current density, electroosmistic permeability, and power consumption were also investigated.