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The effect of nerve growth factor and its antibodies on neurofilament protein expression in primary cultures of sensory and spinal neurons.

Authors
  • Doherty, P
  • Dickson, J G
  • Flanigan, T P
  • Walsh, F S
Type
Published Article
Journal
Neuroscience Letters
Publisher
Elsevier
Publication Date
Sep 28, 1984
Volume
51
Issue
1
Pages
55–60
Identifiers
PMID: 6440067
Source
Medline
License
Unknown

Abstract

An enzyme-linked immunoadsorbent assay has been used to quantify the binding of an antineurofilament monoclonal antibody, RT97, to primary cultures of embryonic chick dorsal root ganglion and spinal cord tissue. In the case of dorsal root ganglion cells a dose-dependent relationship between nerve growth factor (NGF) concentration and the level of RT97 binding was observed. This response could be antagonised by an anti-serum directed against NGF. In contrast, neither NGF nor the antiserum against NGF influenced the binding of RT97 in primary cultures initiated from chick spinal cord.

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