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Effect of neem (Azardirachta indica A. Juss) seeds and leaves extract on some plant pathogenic fungi.

Authors
  • Moslem, M A
  • El-Kholie, E M
Type
Published Article
Journal
Pakistan journal of biological sciences : PJBS
Publication Date
Jul 15, 2009
Volume
12
Issue
14
Pages
1045–1048
Identifiers
PMID: 19947185
Source
Medline
License
Unknown

Abstract

In this study plant pathogenic fungi Alternaria solani, Fusarium oxysporum, Rhizoctonia solani and Sclerotinia sclerotiorum were chosen to study the effect of ethanolic, hexane and methanolic extracts of neem seeds and leaves. Antifungal effects of neem leave and seed extracts obtained by ethanol, hexane and ptrolium ether were examined separately in vitro against Fusarium oxysporum, Rhizoctonia solani, Alternaria solani and Sclerotinia sclerotiorum. Results indicated that seeds and leaves extracts could cause growth inhibition of tested fungi, although the rate of inhibition of tested fungi varied with different extracts and concentrations. But all these extracts and concentrations of extract inhibited the growth of pathogenic fungi at a significant level. Azadirachtin, nimonol and expoxyazdirodione were detected from neem extract by using High Performance Liquid Chromatography (HPLC). We can conclude that neem leave and seed extracts were effective as antifungal against all tested fungi but F. oxysporum and R. solani were the most sensitive fungi.

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