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The effect of near-infrared MLS laser radiation on cell membrane structure and radical generation

Authors
  • Kujawa, Jolanta1
  • Pasternak, Kamila1
  • Zavodnik, Ilya2
  • Irzmański, Robert3
  • Wróbel, Dominika4
  • Bryszewska, Maria5
  • 1 Medical University of Lodz, Clinic of Medical Rehabilitation, 75 Drewnowska Str., Lodz, 91-002, Poland , Lodz (Poland)
  • 2 Grodno State University named after Yanka Kupala, Department of Biochemistry, Blvd Len Kom - 50, Grodno, 230022, Belarus , Grodno (Belarus)
  • 3 Medical University of Lodz, Laboratory of Ergonomics and Exercise Physiology, 1 Haller Sq., Lodz, 90-647, Poland , Lodz (Poland)
  • 4 University of Lodz, Department of General Biophysics, Faculty of Biology and Environmental Protection, 141/143 Pomorska Str., Lodz, 90-237, Poland , Lodz (Poland)
  • 5 University of Lodz, Department of General Biophysics, Faculty of Biology and Environmental Protection, 141/143 Pomorska Str, Lodz, 90-236, Poland , Lodz (Poland)
Type
Published Article
Journal
Lasers in Medical Science
Publisher
Springer-Verlag
Publication Date
Apr 10, 2014
Volume
29
Issue
5
Pages
1663–1668
Identifiers
DOI: 10.1007/s10103-014-1571-y
Source
Springer Nature
Keywords
License
Yellow

Abstract

The therapeutic effects of low-power laser radiation of different wavelengths and light doses are well known, but the biochemical mechanism of the interaction of laser light with living cells is not fully understood. We have investigated the effect of MLS (Multiwave Locked System) laser near-infrared irradiation on cell membrane structure, functional properties, and free radical generation using human red blood cells and breast cancer MCF-4 cells. The cells were irradiated with low-intensity MLS near-infrared (simultaneously 808 nm, continuous emission and 905 nm, pulse emission, pulse-wave frequency, 1,000 or 2,000 Hz) laser light at light doses from 0 to 15 J (average power density 212.5 mW/cm2, spot size was 3.18 cm2) at 22 °C, the activity membrane bound acetylcholinesterase, cell stability, anti-oxidative activity, and free radical generation were the parameters used in characterizing the structural and functional changes of the cell. Near-infrared low-intensity laser radiation changed the acetylcholinesterase activity of the red blood cell membrane in a dose-dependent manner: There was a considerable increase of maximal enzymatic rate and Michaelis constant due to changes in the membrane structure. Integral parameters such as erythrocyte stability, membrane lipid peroxidation, or methemoglobin levels remained unchanged. Anti-oxidative capacity of the red blood cells increased after MLS laser irradiation. This irradiation induced a time-dependent increase in free radical generation in MCF-4 cells. Low-intensity near-infrared MLS laser radiation induces free radical generation and changes enzymatic and anti-oxidative activities of cellular components. Free radical generation may be the mechanism of the biomodulative effect of laser radiation.

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