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Effect of Mycoplasma bovis on expression of inflammatory cytokines and matrix metalloproteinases mRNA in bovine synovial cells.

Authors
  • Nishi, Koji1
  • Gondaira, Satoshi1
  • Okamoto, Mariko1
  • Nebu, Takanori1
  • Koiwa, Masateru2
  • Ohtsuka, Hiromichi2
  • Murai, Kiyokazu3
  • Matsuda, Kazuya4
  • Fujiki, Jumpei5
  • Iwano, Hidetomo5
  • Nagahata, Hajime1
  • Higuchi, Hidetoshi6
  • 1 Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. , (Japan)
  • 2 Department of Veterinary Internal Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. , (Japan)
  • 3 Food Safety and Consumer Affairs Bureau, Ministry of Agriculture Forestry and Fisheries, Kasumigaseki, Tokyo, 100-8950, Japan. , (Japan)
  • 4 Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. , (Japan)
  • 5 Laboratory of Veterinary Biochemistry, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. , (Japan)
  • 6 Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan. Electronic address: [email protected] , (Japan)
Type
Published Article
Journal
Veterinary immunology and immunopathology
Publication Date
Oct 01, 2019
Volume
216
Pages
109920–109920
Identifiers
DOI: 10.1016/j.vetimm.2019.109920
PMID: 31446205
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Mycoplasma bovis causes chronic arthritis in calves. Mycoplasma arthritis shows severe inflammatory reactions in joints that is commonly treated with antibiotics and results in significant economic losses in the calf industry. A previous study showed that inflammatory cytokines and matrix metalloproteinases (MMPs) produced by synovial cells promote progression of the pathophysiology of bacterial arthritis. However, the mechanism underlying the pathogenesis of bovine Mycoplasma arthritis has not been fully clarified. In this study, we examined the immunologic response of bovine synovial tissue to M. bovis. We observed significant increases in expression of interleukin (IL)-1β, IL-6, IL-8, MMP-1, and MMP-3 mRNA in synovial tissue from Mycoplasma arthritis calves compared with tissues from normal calves. Expression of IL-6, IL-8, and MMP-1 mRNA was also induced in cultured synovial cells stimulated with M. bovis, but not expression of IL-1β and MMP-3 mRNA. In contrast, the culture supernatant of peripheral blood mononuclear cells stimulated with M. bovis induced marked increases in the expression of IL-1β, IL-6, IL-8, MMP-1, and MMP-3 mRNA in synovial cells. Our results indicate that inflammatory cytokines and MMPs produced by synovial cells play a key role in the pathogenesis of Mycoplasma arthritis. We suggest that interactions between synovial cells and mononuclear cells in the presence of M. bovis induce expression of these cytokines and MMPs in synovial cells, resulting in severe inflammatory reactions in the joints. Copyright © 2019 Elsevier B.V. All rights reserved.

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