This study evaluates the effect of motivational interviewing with mothers on media interaction and aggression of preschool-aged children. This study was conducted as a quasi-experimental/pre-experimental research in order to evaluate the effect of motivational interviewing with mothers on media interaction and aggression of preschool-aged children. The motivational interviews conducted with the mothers focused on the stages of change and were performed as two face-to-face sessions and two telephone call sessions. While the pre and post-intervention prosocial behavior sub-dimension scores of the children were shown to increase, the overt physical aggression and relational aggression sub-dimension scores were found to decrease. Motivational interviewing technique can be effective for mothers who need to increase control of their children and set limits on media interaction. Nurses in this field can apply the MI technique and help parents to develop behavioral changes.