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The effect of methotrexate on actin and invasiveness of hepatoma Morris 5123 cells in culture.

Authors
  • Otrocka, M
  • Verschueren, H
  • Malicka-Błaszkiewicz, M
Type
Published Article
Journal
Acta biochimica Polonica
Publication Date
Jan 01, 2001
Volume
48
Issue
4
Pages
1051–1060
Identifiers
PMID: 11995967
Source
Medline
License
Unknown

Abstract

Monomeric (G), total (T) and filamentous (F) actin and the state of actin polymerisation (F:G) were determined and actin filaments were visualized in hepatoma Morris 5123 cells cultured in the presence of methotrexate (MTX) at various concentration. The exposure of the cells to this drug resulted in a decrease of total and polymerised actin in cytoplasm and in some changes in actin filament organization. This coincided with a decrease of the cells' ability to migrate through Matrigel coated filters and with inhibition of tumour formation after reimplantation of the methotrexate treated cells to experimental rats.

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