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The effect of melatonin on testis histological changes and spermatogenesis indexes in mice following treatment with dexamethasone.

Authors
  • Soleimani Mehranjani, Malek1
  • Azizi, Mina1
  • Sadeghzadeh, Firouzeh1
  • 1 Department of Biology, Faculty of Science, Arak University, Arak, Iran. , (Iran)
Type
Published Article
Journal
Drug and Chemical Toxicology
Publisher
Informa UK (Taylor & Francis)
Publication Date
May 01, 2022
Volume
45
Issue
3
Pages
1140–1149
Identifiers
DOI: 10.1080/01480545.2020.1809672
PMID: 33161762
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Dexamethasone is a common medicine that is capable of causing malformation in the male reproductive system. The aim of this study was to investigate the effect of melatonin on testis histological changes and Spermatogenesis indexes in adult mice following treatment with dexamethasone. Adult male NMRI mice were divided randomly into four groups: control, dexamethasone (i.p injections, 7 mg/kg/day), dexamethasone + melatonin and melatonin (i.p injections, 20 mg/kg/day). After 7 days of treatment, the right testes were studied stereologically and the left testes were used to measure the daily sperm production (DSP). The serum levels of malondialdehyde (MDA), testosterone and total antioxidant capacity (TAC) were also measured. The left caudal epididymis was used to analyze sperm parameters. Data were analyzed using one way ANOVA and means were considered significantly different at p < 0.05. A significant decrease in the testis volume, seminiferous tubules volume, the number of spermatocytes, round and long spermatids, Spermatogenesis indexes, sperm parameters such as motility, count, viability, tail length and DSP, serum testosterone level, TAC and the body weight was found in the dexamethasone group compared to the control. Meanwhile a significant swelling of the interstitial tissue and a significant increase in the MDA level was found in the dexamethasone group compared to the control. The above parameters reached the control level in the dexamethasone + melatonin group. Melatonin can compensate for the adverse effects of dexamethasone on sperm parameters and the histology of the seminiferous tubules in mice.

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