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Effect of medium components and time of cultivation on chitin production by Mucor circinelloides (Mucor javanicus IFO 4570) -- a factorial study.

Authors
  • Sousa Andrade, Vânia
  • de Barros Neto, Benicío
  • Fukushima, Kazutaka
  • Campos Takaki, Galba Maria de
Type
Published Article
Journal
Revista iberoamericana de micología
Publication Date
Dec 01, 2003
Volume
20
Issue
4
Pages
149–153
Identifiers
PMID: 15456353
Source
Medline
License
Unknown

Abstract

Chitin production of mycelia from Mucor circinelloides (Mucor javanicus) was studied with a two-level factorial design in all combinations of the following factors in the culture medium: time of cultivation, concentration of D-glucose, L-asparagine and thiamine. Chitin was characterised by infrared and nuclear magnetic resonance spectroscopy. The four factors showed statistically significant (95%) positive main effects on chitin production, without interactions between them. The highest chitin yield (23.9%) was obtained with the medium containing 60 g/l of glucose, 3 g/l of asparagine and 0.008 mg/l of thiamine and is comparable to the highest in the literature.

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