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The effect of a mannose binding protein on macrophage interactions with Candida albicans.

Authors
  • Kitz, D J
  • Stahl, P D
  • Little, J R
Type
Published Article
Journal
Cellular and molecular biology
Publication Date
Jul 01, 1992
Volume
38
Issue
4
Pages
407–412
Identifiers
PMID: 1499040
Source
Medline
License
Unknown

Abstract

A soluble mannose binding protein (MBP), obtained from rabbit serum, was found to inhibit phagocytosis of Candida albicans by bone marrow derived, cultured murine macrophages. During in vitro incubation of yeast with lymphocyte-free macrophage populations uptake of the yeast was significantly reduced at MBP concentrations of 5 micrograms/ml. A similar reduction in yeast phagocytosis was produced by dextrose, d-fucose, l-fucose, d-mannose and alpha-methyl-d-mannoside but required saccharide concentrations of 25-50 mg/ml. Inhibition of phagocytosis of the yeast also resulted from pretreatment of either the macrophages or the yeasts with MBP followed by washing. As expected, the addition of mannan to the assay medium blocked the inhibitory effect of MBP for uptake of C. albicans. These findings suggest that both cell bound and soluble mannose receptors may be important modulators of macrophage-Candida interactions.

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