This paper describes the He non-thermal plasma treatment of liquid impregnated cotton fabrics. The cotton fabrics were soaked in either H2O, D2O or ethanol after which they were placed in an atmospheric pressure parallel-plate DBD reactor. The influence of the used liquid in combination with the plasma exposure time was studied using OES, FTIR, XPS and SEM. The addition of (deuterated) water was found to aid more efficiently in the incorporation of polar functional groups onto the cotton surface in comparison to a pure He plasma treatment. The presence of H and OH species in the discharge also caused extensive etching of the surface, which led to the formation of microcraters. The impregnation with ethanol was responsible for the formation of a plasma-based thin film on top of the cotton substrate, which was characterized by a high content of C-C bonds and a smooth surface morphology. These results show that the soaking of cotton fabric prior to plasma exposure can help to more effectively alter its surface properties compared to a dry plasma treatment.