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The effect of interleukin 17 and Toll-like receptor 2 on CD11b expression and apoptosis of neutrophils in zymosaninduced arthritis and paw oedema.

Authors
  • Milanova, Viktoriya
  • Ivanovska, Nina
  • Dimitrova, Petya
Type
Published Article
Journal
Central-European journal of immunology / Polish Society for Immunology and eleven other Central-European immunological societies
Publication Date
Jan 01, 2014
Volume
39
Issue
2
Pages
131–141
Identifiers
DOI: 10.5114/ceji.2014.43712
PMID: 26155114
Source
Medline
Keywords
License
Unknown

Abstract

We investigated the effect of interleukin (IL)-17 on Ly6G(+) cell apoptosis in zymosan-induced arthritis (ZIA) and oedema (ZIO). Zymosan injection at the ankle joint caused swelling and coincided with histological joint alterations and IL-17A expression in areas with cell infiltrates. Flow cytometry of blood demonstrated increased frequencies of Ly6G(+)CD11b(+) cells and their decreased apoptosis in ZIA. Annexin V(+) neutrophils had lower CD11b expression, unlike Annexin V(-) cells. Cell survival for 12 hours was affected neither by IL-17 nor by zymosan alone, while both stimuli diminished Annexin V(+) cell frequencies and up-regulated CD11b on Annexin V(-) cells. Interleukin 17 antagonised to the effects of zymosan in 24-hour cultures. The administration of IL-17 in ZIO increased paw thickness, enlarged the blood Ly6G(+) pool, elevated CD11b expression and decreased apoptosis. We suggest that altered neutrophil apoptosis in arthritis can be overcome by anti-IL-17 therapy combined with an inhibition of Toll-like receptor (TLR) 2 and CD11b signalling.

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