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The Effect of Human Growth Hormone on Superoxide Dismutase Activity, Glutathione and Malondialdehyde Levels of Hypoxic-Ischemic Newborn Rat Brain

Authors
  • Yapıcıoğlu, Hacer
  • Satar, Mehmet
  • Canacankatan, Necmiye
  • Tutak, Ercan
  • Sertdemir, Yaşar
  • Antmen, Efsun
  • Narlı, Nejat
Type
Published Article
Journal
Neonatology
Publisher
S. Karger AG
Publication Date
Sep 22, 2006
Volume
90
Issue
3
Pages
168–173
Identifiers
DOI: 10.1159/000092680
PMID: 16636532
Source
Karger
Keywords
License
Green
External links

Abstract

Objectives: We investigated the effect of human growth hormone (GH) on newborn rat brain superoxide dismutase, glutathione and malondialdehyde (MDA) levels in hypoxic-ischemic (H-I) newborn rats. Methods: Fourty-eight 7 days old newborn rats were randomized to a healthy (n: 15), H-I (n: 18) and GH administered H-I (GH-H-I, n: 15) group. Permanent, left common carotid ligation was performed in the H-I groups. In the GH-H-I group, 50 mg/kg human GH (Norditropin Simplex, Novo Nordisk A/S) was administered subcutaneously just before carotid artery ligation. Two hours after ligation, rats were subjected to 2 h of hypoxemia and then were decapitated. Right and left cerebral hemispheres (CHs) and cerebellum-brain stem (C-BS) were separated. Results: Glutathione levels of each region were not statistically different from each other in and between the groups. Superoxide dismutase levels were higher in C-BSs compared to CHs (for each comparison p < 0.01). CHs and C-BS MDA levels were similar in the control and H-I groups but MDA levels of both CHs of the GH-H-I group were significantly higher than the levels of the H-I group (p = 0.01; p = 0.024, respectively). Left CH MDA level of GH-H-I group was higher compared to left CH MDA of the control group (p = 0.045) while there was no difference between right CHs. In the GH-H-I group, left CH MDA level was higher than the C-BS (p = 0.03). MDA levels of the C-BSs did not differ between the groups (p > 0.05). Conclusion: Although we have not evaluated the effect of GH histopathologically, increased lipid peroxidation especially in the H-I (left) hemisphere of the GH treated rats might suggest that GH treatment may be harmful in H-I encephalopathy.

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