The present investigations serve to characterize Inconel 718 layers deposited onto a stainless steel by means of wire-based electron beam cladding. The investigations analyze the depth-dependent element concentration and the hardness profile, while providing a detailed microstructural examination. It is thus shown that multilayer coating systems can be applied without defects, and that the chemical composition of the additive material can be achieved in the third layer. Furthermore, the Fe content is shown to have a significant influence on the segregation of the Laves phase during solidification. The investigations also analyze the necessity of a subsequent solution annealing heat treatment step by means of hardness mapping and microstructural investigations. As a result, it is evident that solution annealing at 1050 °C reduces the amount of the Laves phase, but does not completely dissolve it. Additional age hardening tests with different T-t variants yield the desired hardness range. As a result, these variants offer a suitable possibility for in situ age hardening during boriding tests planned for future research.