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Effect of heat processing on the chemical constituents and NO-suppressing activity of Bletilla Tuber.

Authors
  • Nishidono, Yuto1
  • Ishii, Toshinari2
  • Okada, Ryo2
  • Norimoto, Hisayoshi3
  • Murayama, Chiaki3
  • He, Dingxiang4
  • Okuyama, Tetsuya2
  • Nishizawa, Mikio2
  • Tanaka, Ken5
  • 1 College of Pharmaceutical Sciences, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, 525-8577, Shiga, Japan. , (Japan)
  • 2 Department of Biomedical Sciences, College of Life Sciences, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, 525-8577, Shiga, Japan. , (Japan)
  • 3 PuraPharm Japan Corporation, Room 406, 4th FL., JOHO BLDG., 527 Takata, Toyama, 930-0866, Japan. , (Japan)
  • 4 Gold Sparkle (Guizhou) DZ Chinese Medicine Co., Ltd, Danzhai, 557500, China. , (China)
  • 5 College of Pharmaceutical Sciences, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, 525-8577, Shiga, Japan. [email protected] , (Japan)
Type
Published Article
Journal
Journal of natural medicines
Publication Date
Nov 13, 2019
Identifiers
DOI: 10.1007/s11418-019-01371-y
PMID: 31720996
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Bletilla Tuber (dried tuber of Bletilla striata) is used as an astringent hemostatic medicine for the treatment of ulcers, bleeding, and burns in traditional Chinese medicine (TCM). The Chinese Pharmacopoeia describes the heat processing methods used on raw tubers of Bletilla striata to produce the herbal medicine "Bletilla Tuber". In this study, we compared the chemical constituents of well-processed Bletilla Tuber (BT1) and normally processed Bletilla Tuber (BT2) derived from the same origin. In addition, as an indicator of the hemostatic activity of Bletilla Tuber, the NO inhibitory activities of extracts obtained from BT1 and BT2 and the isolated compounds were examined. As a result of LC-MS analysis, three types of compounds, glucosyloxybenzyl 2-isobutylmalates, bibenzyl derivatives and phenanthrene derivatives, were detected. Comparison of the chemical profiles of the extracts indicated that the relative contents of glucosyloxybenzyl 2-isobutylmalates had changed by heat processing, whereas the relative contents of bibenzyls and phenanthrenes had not changed. The extracts of BT1 and BT2 showed similar IC50 values on NO production suppressing activity. Furthermore, phenanthrenes and bibenzyls were identified as the compounds responsible for suppressing the NO activity. These results suggest that the biological activities, such as the anti-inflammatory and hemostatic activities, of Bletilla Tuber are not affected by heat processing.

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