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Effect of hCG application at different moments of the estrous cycle on corpus luteum and uterine vascularization and serum progesterone concentration in mares

Authors
  • Alonso, Maria Augusta1
  • Silva, Luciano Andrade2
  • Affonso, Fernanda Jordão1
  • Lemes, Kleber Menegon1
  • Celeghini, Eneiva Carla Carvalho1
  • Lançoni, Renata1
  • Carvalho, Henrique Fulanetti1
  • de Arruda, Rubens Paes1
  • 1 Laboratory of Semen Biotechnology and Andrology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo (USP), Pirassununga, São Paulo, Brazil.
  • 2 Laboratory of Theriogenology Dr. O.J. Ginther, Department of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga, São Paulo, Brazil.
Type
Published Article
Journal
Animal Reproduction
Publisher
Colégio Brasileiro de Reprodução Animal - CBRA
Publication Date
Oct 24, 2019
Volume
16
Issue
2
Pages
317–327
Identifiers
DOI: 10.21451/1984-3143-AR2018-0103
PMID: 33224293
PMCID: PMC7673598
Source
PubMed Central
Keywords
Disciplines
  • Original Article
License
Green

Abstract

Establishment of pregnancy after embryo transfer is the ultimate goal of an embryo transfer program and increasing pregnancy rates and reducing pregnancy loss are mandatory. The utilization of treatments to improve conception rates in recipient mares has been the focus of several research groups over the last years and the results are controversial. Some studies using human chorionic gonadotrophin (hCG) found promising results. Our hypothesis was that hCG administration would cause an additional stimulation on luteal function, uterine and luteal vascularization and progesterone concentration, and the mares would have increased uterine and cervix tone. Therefore, in the present study the effects of hCG administration to induce ovulation, on day 0 (day of ovulation) or day 5 postovulation were evaluated on corpus luteum characteristics, reproductive tract vascularization, and serum progesterone concentration from ovulation until day 15 postovulation. Groups were: G1: (control) - no hCG; G2: 2500 IU of hCG to induce ovulation when a follicle greater than 35mm and uterine edema were detected; G3: 2500 IU hCG on day 0; G4: 2500 IU hCG on day 5 postovulation. Twelve mares were randomly assigned to each group, during consecutive cycles, in a Latin Square experimental design, in a total of 48 cycles. Doppler ultrasound evaluations were performed daily from day 0 until day 15 postovulation, including mesometrial vascularity, endometrial vascularity and corpus luteum vascularity. Blood samples were collected for serum progesterone concentration. Data was analyzed using the Proc Glimmix SAS Procedure for nonparametric variables and Proc Mixed for parametric parameters. There was no treatment effect for all variables studied (P > 0.05). Characteristics were only affected by day (P < 0.05). It can be concluded that hCG administration at the time points suggested in the current study did not alter the characteristics evaluated.

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