Affordable Access

deepdyve-link
Publisher Website

The effect of graphene oxide on signalling of xenobiotic receptors involved in biotransformation.

Authors
  • Ženata, Ondřej1
  • Vrzalová, Aneta1
  • Bachleda, Petr2
  • Janečková, Jana2
  • Panáček, Aleš3
  • Kvítek, Libor3
  • Vrzal, Radim4
  • 1 Department of Cell Biology and Genetics, Faculty of Science, Palacky University in Olomouc, Slechtitelu 27, Olomouc, CZ-783 71, Czech Republic. , (Czechia)
  • 2 Department of Surgery, University Hospital, I.P. Pavlova 6, 775 15, Olomouc, Czech Republic. , (Czechia)
  • 3 Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacky University in Olomouc, 17. listopadu 12, 771 46, Olomouc, Czech Republic. , (Czechia)
  • 4 Department of Cell Biology and Genetics, Faculty of Science, Palacky University in Olomouc, Slechtitelu 27, Olomouc, CZ-783 71, Czech Republic. Electronic address: [email protected] , (Czechia)
Type
Published Article
Journal
Chemosphere
Publication Date
Aug 01, 2020
Volume
253
Pages
126753–126753
Identifiers
DOI: 10.1016/j.chemosphere.2020.126753
PMID: 32464781
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Graphene oxide (GO) is an engineered nanomaterial which was demonstrated to have outstanding capacity for adsorption of organic pollutants such as polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), the ligands and activators of the aryl hydrocarbon receptor (AhR). Due to the partially overlapping ligand capacity of AhR and pregnane X receptor (PXR), we tested the impact of GO particles on their signalling. While reporter gene assay revealed potentiating effect of GO on ligand-activated AhR-dependent luciferase activity, there was no effect for PXR. However, inducible target genes for AhR (CYP1A1) or PXR (ABCB1) were decreased at mRNA as well as protein levels by the presence of GO in HepG2 (for AhR), LS180 (for PXR) or primary human hepatocytes (both receptors). Moreover, the presence of GO diminished PXR and AhR protein levels in primary cultures of human hepatocytes. This was partially reversed by proteasome inhibitor MG132 for AhR but not for PXR. In conclusion, GO decreases ligand-stimulated activities of AhR and PXR in human cells. Copyright © 2020 Elsevier Ltd. All rights reserved.

Report this publication

Statistics

Seen <100 times