We have successfully constructed the eukaryotic expression vector pEGFR-c3-GPC3, which allows stable GPC3 expression in MHCC97-L/GPC3 cells. The upregulation of GPC3 expression can stimulate the growth of hepatoma cell line MHCC97-L in vitro.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/21421480