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The effect of ginger extract on glycoproteins of Raji cells.

Authors
  • Zamani, Zahra
  • Kohan, Haleemeh Kabini
  • Kadivar, Mehdi
  • Kalyee, Zahra
  • Rad, Behzad Laame
  • Iravani, Ayda
  • Rahimi, Nourooz Ali
  • Wahabi, Farideh
  • Sadeghi, Sedigheh
  • Pourfallah, Fatemeh
  • Arjmand, Mohammad
Type
Published Article
Journal
Pakistan journal of biological sciences : PJBS
Publication Date
Jan 15, 2014
Volume
17
Issue
2
Pages
241–247
Identifiers
PMID: 24783808
Source
Medline
License
Unknown

Abstract

Protein glycosylation is associated with the development and progression of specific diseases, including cancers. The ginger rhizome is known to have anti-cancer and anti-fungal properties. This investigation was carried out to study the effect of ginger on glycoproteins of Raji cells. A 10% yield of ginger extract was mixed with 0.01% DMSO and added to 6 x 10(4) Raji cells at different concentrations for 24, 48 and 72 h at 37 degrees C. Their half maximal inhibitory concentration (IC50) was determined and analyzed statistically using Graphpad prism software. Cell extracts were prepared and their glycoproteins purified using lectin-affinity chromatography (Q proteome total glycoprotein and O glycoprotein kits) and SDS PAGE was carried out. IC50 of ginger extract on Raji cells was 20 microg mL(-1) at 72 h with < 0.01 significance. Silver staining of purified glycoprotiens in Raji cells indicated the presence of O-glycans and N-glycans. N-linked mannose and N-linked sialic acids were detected with the total glycoprotein kit. O-linked galactose and O-linked sialic acids were identified with the O-glycoprotein. Ginger reduced the expression of O-linked sialic acid and also N-linked mannose on Raji cells but had no effect on other glycoproteins. Sialic acid is now well known as a cancer marker and investigations are on to use it as a drug-target in cancerous tissues.

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