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Effect of Fe 2+-induced lipid peroxidation upon microsomal steroidogenic enzyme activities of porcine adrenal cortex

Authors
  • Imataka, Hiroaki
  • Suzuki, Keiko
  • Tamaoki, Bun-ichi
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publication Date
Jan 01, 1985
Volume
128
Issue
2
Pages
657–663
Identifiers
DOI: 10.1016/0006-291X(85)90096-8
Source
Elsevier
Keywords
License
Unknown

Abstract

When porcine adrenocortical microsomes were treated with Fe 2+, enhanced production of malondialdehyde was observed as a result of membrane lipid peroxidation. By treatment of the microsomes with Fe 2+, the activity of Δ 5-3β-hydroxysteroid dehydrogenase coupled with Δ 5-Δ 4 isomerase, concentration of cytochrome P-450 and the activity of the cytochrome-involving enzyme systems such as 17α- and 21-hydroxylases were significantly reduced. 17α-Hydroxylase activity was more effectively decreased by Fe 2+ than that of 21-hydroxylase. On the other hand, activity of NADH- and NADPH-cytochrome c reductases remained unchanged or somewhat increased. Both the induction of lipid peroxidation and the decrease of the enzyme activities were prevented by α-tocopherol and N,N′-diphenyl- p-phenylenediamine.

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