Affordable Access

[Effect of endothelin on isolated myocytes from rat heart].

Authors
  • Tang, C S
  • Li, Z P
  • Fan, G
  • Tang, J
  • Su, J Y
Type
Published Article
Journal
Sheng li xue bao : [Acta physiologica Sinica]
Publication Date
Feb 01, 1992
Volume
44
Issue
1
Pages
15–21
Identifiers
PMID: 1317972
Source
Medline
License
Unknown

Abstract

The mechanism of endothelin (ET) action on isolated myocytes of rat heart was investigated. Administration of 10(-9)-10(-7) mol/L ET caused dose-dependently severe contracture of myocytes, increase in cytosolic lactate dehydrogenase leakage and increase of cellular total calcium. ET also enhanced 45Ca2+ influx in myocytes, that could be antagonized by calcium channel blocker verapamil. Incubation of Fura-2 -preloaded myocytes with ET would induce a significant increase of cytosolic free Ca2+, and this effect of ET could also be partly antagonized by verapamil. These results suggest that ET enhances Ca2+ influx in myocytes mainly through voltage-dependent calcium channel, by which ET exerts its biological action.

Report this publication

Statistics

Seen <100 times