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Effect of emulsan on biodegradation of crude oil by pure and mixed bacterial cultures.

Authors
  • Foght, J M
  • Gutnick, D L
  • Westlake, D W
Type
Published Article
Journal
Applied and environmental microbiology
Publication Date
Jan 01, 1989
Volume
55
Issue
1
Pages
36–42
Identifiers
PMID: 16347832
Source
Medline
License
Unknown

Abstract

Crude oil was treated with purified emulsan, the heteropolysaccharide bioemulsifier produced by Acinetobacter calcoaceticus RAG-1. A mixed bacterial population as well as nine different pure cultures isolated from various sources was tested for biodegradation of emulsan-treated and untreated crude oil. Biodegradation was measured both quantitatively and qualitatively. Recovery of CO(2) from mineralized C-labeled substrates yielded quantitative data on degradation of specific compounds, and capillary gas chromatography of residual unlabeled oil yielded qualitative data on a broad spectrum of crude oil components. Biodegradation of linear alkanes and other saturated hydrocarbons, both by pure cultures and by the mixed population, was reduced some 50 to 90% after emulsan pretreatment. In addition, degradation of aromatic compounds by the mixed population was reduced some 90% in emulsan-treated oil. In sharp contrast, aromatic biodegradation by pure cultures was either unaffected or slightly stimulated by emulsification of the oil.

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