The study was carried out on 40 ostriches randomly allocated into five groups and each group was randomly assigned to a different dietary treatment. Experimental diets were made on the basis of a control diet, where a part of the gross energy was replaced with energy from linseed (4 or 8% -treatment L4 and L8, respectively) or rapeseed (5 or 10% - treatment R5 and R10, respectively). The ostriches were slaughtered at 12 months of age and within 30 minutes after slaughter liver samples (50 g) were taken. The results indicate that the linseed supplementation, especially 4%, to the ostrich diet improves the nutritional value of ostrich liver by decreasing the n-6/n-3 ratio and by increasing the PUFA/SFA ratio. It makes ostrich liver more valuable for the production of meat products e.g. pates. The rapeseed supplementation to the ostrich diet has no influence on the fatty acid profile of ostrich liver compared to the control diet. Dietary supplementation by linseed changes the fatty acid metabolism in ostrich liver.