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Effect of dietary antioxidants on the cytostatic effect of acrylamide during copper-deficiency in Saccharomyces cerevisiae.

Authors
  • Kommuguri, Upendra Nadh
  • Satyaprasad Pallem, Poorna Venkata
  • Bodiga, Sreedhar
  • Bodiga, Vijaya Lakshmi
Type
Published Article
Journal
Food & Function
Publisher
The Royal Society of Chemistry
Publication Date
Apr 01, 2014
Volume
5
Issue
4
Pages
705–715
Identifiers
DOI: 10.1039/c3fo60483g
PMID: 24526248
Source
Medline
License
Unknown

Abstract

Acrylamide exposure increases oxidative stress and causes cytotoxicity. In order to understand the role of oxidative stress in acrylamide toxicity, we utilized Saccharomyces cerevisiae as a model organism grown in Yeast Peptone Dextrose (YPD) or Copper-Deficient Medium (CDM). Although the growth curves of yeast were comparable in these media, acrylamide treatment resulted in significant growth inhibition and colony formation only in the CDM. Copper-deficiency induced a decrease in the intracellular metallothionein levels, along with reduced Cu, Zn-SOD activity that appeared to increase the sensitivity of the yeast to the cytostatic effect of acrylamide. Increased dichlorofluorescein (DCF) fluorescence, enhanced formation of para-phenyl tertiary butyl nitrone (PBN)-hydroxyethyl adducts and a lowered reduced glutathione (GSH) content were observed under copper-deficient conditions, when challenged with acrylamide. The cytostatic effects and intracellular redox changes in response to acrylamide were ameliorated by antioxidant molecules viz. a viz. curcumin, β-carotene, vanillin and caffeic acid, which effectively decreased the oxidative stress and improved the growth recovery.

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