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Effect of common bean seed exudates on growth, lipopolysaccharide and lipopolysaccharide transport gene expression of Rhizobium anhuiense.

Authors
  • Xiang, Quanju1
  • Wang, Jie2
  • Qin, Peng3
  • Adil, Bilal4
  • Xu, Kaiwei5
  • Gu, Yunfu6
  • Yu, Xiumei7
  • Zhao, Ke8
  • Zhang, Xiaoping9
  • Ma, Menggen10
  • Chen, Qiang11
  • Chen, Xiaoqiong12
  • Yan, Yanhong13
  • 1 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource , No.211 , Huimin Road , Wenjiang District , Chengdu, Sichuan, China , 611130 ; [email protected] , (China)
  • 2 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource, Chengdu, Sichuan, China ; [email protected] , (China)
  • 3 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource, Chengdu, Sichuan, China ; [email protected] , (China)
  • 4 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource, Chengdu, Sichuan, China ; [email protected] , (China)
  • 5 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource, Chengdu, Sichuan, China ; [email protected] , (China)
  • 6 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource, Chengdu, Sichuan, China ; [email protected] , (China)
  • 7 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource, Chengdu, Sichuan, China ; [email protected] , (China)
  • 8 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource, Chengdu, Sichuan, China ; [email protected] , (China)
  • 9 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource, Chengdu, Sichuan, China ; [email protected] , (China)
  • 10 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource, Chengdu, Sichuan, China ; [email protected] , (China)
  • 11 Sichuan Agricultural University - Chengdu Campus, 506176, College of Resource, Chengdu, Sichuan, China ; [email protected] , (China)
  • 12 Sichuan Agricultural University - Chengdu Campus, 506176, Rice Research Institute, Chengdu, Sichuan, China ; [email protected] , (China)
  • 13 Sichuan Agricultural University - Chengdu Campus, 506176, College of Animal Science and Technology, Chengdu, Sichuan, China ; [email protected] , (China)
Type
Published Article
Journal
Canadian Journal of Microbiology
Publisher
Canadian Science Publishing
Publication Date
Nov 21, 2019
Identifiers
DOI: 10.1139/cjm-2019-0413
PMID: 31751146
Source
Medline
Language
English
License
Unknown

Abstract

Lipopolysaccharide (LPS) is essential for successful nodulation during the symbiosis of rhizobia and legumes. However, the detailed mechanism of the LPS in this process has not yet been clearly elucidated. In this study, the effects of exudates the common bean seeds on the growth, lipopolysaccharide production and lipopolysaccharide transport genes expression (lpt) of Rhizobium anhuiense were investigated. R. anhuiense exposed to exudates showed changes in LPS electrophoretic profiles and content, where the LPS band was wider and the LPS content was higher in R. anhuiense treated with seeds exudates. Exudates enhanced cell growth of R. anhuiense in a concentration dependent manner; R. anhuiense exposed to higher doses of the exudate showed faster growth. Seven ltp genes of R. anhuiense were amplified and sequenced. Sequences of six lpt genes, except for lptE, were the same as found in previously analyzed R. anhuiense strains, while lptE shared low sequence similarity with other strains. Exposure to the exudates strongly stimulated the expression of all lpt genes. An approximately 6.7- (lptG) to 301-fold (lptE) increase in the transcriptional levels were observed after only 15 min of exposure to exudates. These results indicate that seed exudates affect the LPS by making the cell wall structure more conducive to symbiotic nodulation.

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