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The effect of chronic alcohol feeding on lipid peroxidation in microsomes: Lack of relationship to hydroxyl radical generation

Authors
  • Shaw, Spencer
  • Jayatilleke, Elizabeth
  • Lieber, Charles S.
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publication Date
Jan 01, 1984
Volume
118
Issue
1
Pages
233–238
Identifiers
DOI: 10.1016/0006-291X(84)91091-X
Source
Elsevier
Keywords
License
Unknown

Abstract

Chronic alcohol feeding causes microsomal induction including increased generation of hydroxyl radicals. Ethanol induced liver injury may be mediated by lipid peroxidation for which hydroxyl radicals have been proposed as major mediators. Ethanol promotes lipid peroxidation when given acutely but also may serve as a hydroxyl radical scavenger. Therefore, we studied the acute and chronic effects of alcohol on microsomal lipid peroxidation and hydroxyl radical generation. Chronic alcohol feeding in rats increased microsomal generation of hydroxyl radicals but lipid peroxidation of endogenous lipid was inversely related to hydroxyl radical generation. Ethanol (50mM) had a slight inhibitory effect on hydroxyl radical production in perxidizing microsomes, no effect on endogenous lipid peroxidation and enhanced the lysis of RBCs added as targets of peroxidation. Enhanced microsomal generation of hydroxyl radicals following chronic alcohol feeding is not an important mediator of lipid peroxidation.

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