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Effect of buffer pH and peptide composition on the selectivity of peptide separations by capillary zone electrophoresis.

Authors
Type
Published Article
Journal
Analytical biochemistry
Publication Date
Volume
173
Issue
2
Pages
265–270
Identifiers
PMID: 3189808
Source
Medline

Abstract

A series of 10 synthetic peptides containing varying degrees of charge and hydrophobicity was used to study the effects of peptide composition and buffer pH on the selectivity of separations by capillary zone electrophoresis (CZE). A simple model is used to explain the effect of buffer pH on the separation. It was found that pH is an important parameter affecting the selectivity of CZE separations. Furthermore, it is shown that the selectivity of the separation is such that peptides differing in neutral amino acid composition can be resolved, and that even differences in a peptide's amino acid sequence can be detected. A protease digest of beta-lactoglobulin A is shown as a practical example of a separation of a complex peptide mixture.

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