Affordable Access

deepdyve-link
Publisher Website

The effect of bee bread (Perga) with chemotherapy on MDA-MB-231 cells.

Authors
  • Caner, Armagan1, 2
  • Onal, Muge Gulcihan3, 4
  • Silici, Sibel5
  • 1 Department of Biophysics, Faculty of Medicine, Erciyes University, Kayseri, Turkey. [email protected] , (Turkey)
  • 2 Genome and Stem Cell Center, Erciyes University, Kayseri, Turkey. [email protected] , (Turkey)
  • 3 Genome and Stem Cell Center, Erciyes University, Kayseri, Turkey. , (Turkey)
  • 4 Halil Bayraktar Health Vocational College, Erciyes University, Kayseri, Turkey. , (Turkey)
  • 5 Department of Agricultural Biotechnology, Faculty of Agriculture, Erciyes University, Kayseri, Turkey. , (Turkey)
Type
Published Article
Journal
Molecular Biology Reports
Publisher
Springer-Verlag
Publication Date
Mar 15, 2021
Identifiers
DOI: 10.1007/s11033-021-06259-3
PMID: 33721171
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Bee bread (BB) is a bee product like propolis and honey. It is the main food for larvae and bees producing royal jelly in the hive. It also known as Perga. As with other bee products, it is increasingly popular due to its antioxidant properties. The aim of this study was to examine the effects of BB on MDA-MB-231 breast cancer cells and the effects on these cells when administered together with Doxorubicin (DOX) and Cisplatin (CDDP), used in cancer treatment. The proliferation of the cells was determined by applying 5 mg/mL BB together with different concentrations of DOX and CDDP. In addition to these studies, the effect of DOX+BB and CDDP+BB combinations on the migration of MDA-MB-231 cells was determined by the wound healing method. The expression levels of Bid and Bcl-2 were determined by RtqPCR. According to these studies, as expected, BB did not show a significant toxic effect on MDA-MB-231 cells at different concentrations. BB significantly suppressed the effect of DOX and CDDP on the proliferation of MDA-MB-231 cells. BB with DOX and CDDP suppressed the proapoptotic Bid gene while overexpressing the anti-apoptotic Bcl-2 gene, separately. Interestingly, BB blocked the migration of MDA-MB-231 cells by 50% even after 72 h. As a result, BB significantly reduced the toxicity of DOX and CDDP on MDA-MB-231 cells. The most interesting result of the study is that BB prevented the migration of cancer cells.

Report this publication

Statistics

Seen <100 times