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The effect of automobile exhaust particulates on cell viability, plating efficiency and cell division of mammalian tissue culture cells.

Authors
  • Seemayer, N H
  • Hadnagy, W
  • Tomingas, R
Type
Published Article
Journal
The Science of The Total Environment
Publisher
Elsevier
Publication Date
Mar 01, 1987
Volume
61
Pages
107–115
Identifiers
PMID: 2437649
Source
Medline
License
Unknown

Abstract

Extract of particulate matter (EPM) of gasoline engine exhaust induced only a slight loss of cell viability of mouse macrophages (line IC-21) in vitro, while a strong dose-dependent reduction of plating efficiency of human cell line A-549 and of Syrian hamster line 14-1b occurred. Cytological investigations of exposed macrophages of line IC-21 revealed an increase in the mitotic index from 1.5% of control values up to 14.6% at the highest tested concentration of EPM. Mitotic arrest is based almost exclusively on C-type mitoses occurring dose-dependently in the presence of EPM. Results indicate disturbances of the spindle apparatus in the presence of EPM.

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