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The effect of aspirin on the metabolism of exogenous arachidonic acid in human polymorphonuclear leukocytes.

Authors
  • Punnonen, K
  • Uotila, P
Type
Published Article
Journal
Prostaglandins, leukotrienes, and medicine
Publication Date
Aug 01, 1984
Volume
15
Issue
2
Pages
177–185
Identifiers
PMID: 6436829
Source
Medline
License
Unknown

Abstract

When human polymorphonuclear leukocytes (PMNL) were incubated with exogenous 14C-arachidonic acid (14C-AA), both lipoxygenase and cyclo-oxygenase metabolites were detected. The amount of the 5-lipoxygenase metabolites formed, including 5-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE), was small. The amount of other mono-HETE's (migrating in the vicinity of 12-HETE) was greater, but this was obviously mainly due to the small amount of contaminating platelets. In the presence of calcium ionophore A23187 the rate of formation of 5-HETE was increased, but the formation of other metabolites remained unchanged. When PMNL were incubated with aspirin in the presence of A23187 the formation of the cyclo-oxygenase products was decreased but that of 5-HETE was unchanged. The present study indicates that the calcium ionophore A23187 stimulates specifically the 5-lipoxygenase in human PMNL and that aspirin has no effect on the formation of the 5-lipoxygenase metabolites of arachidonic acid in human PMNL.

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