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Efeitos da suplementação com semente de girassol em fêmeas bovinas na produção de oócitos e embriões in vitro

Authors
  • Baltazar, Angélica Leão
Publication Date
Jan 15, 2016
Source
Repositório Institucional UNESP
Keywords
Language
Portuguese
License
Unknown
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Abstract

Supplementation with compounds rich in linoleic acid, among which is included sunflower seed, promotes increase in conception rates in cows. It was hypothesized that sunflower seed supplementation in oocyte donors increases the number and quality of oocytes, increases the cleavage rates and determines an increase in the number and quality of blastocysts produced in vitro. Therefore the objective was to investigate the effect of such supplementation, in the number and quality of oocytes cultured in vitro on cleavage rate and in the number and quality of in vitro produced blastocysts. Therefore, cows five and twenty-five heifers (n = 30) Nellore were divided into two groups to receive one of the following treatments: 1.7 kg / day supplement containing 53% soybean meal 44% and 47% PB corn (Control Group - Group C, n = 15) or 1.7 kg / containing 40% add-on of soybean meal with 44% crude protein (CP) and 60% sunflower seed (Sunflower group - Group C; n = 15) for 57 days. Females underwent follicular aspiration on days D0, D13, D29, D43, D56 and D77 (D0 = start of supplementation; D56 = end of supplementation). The oocytes were subjected to in vitro embryo production process. Data were analyzed by the Mixed procedure (SAS) version 9.2, by ANOVA mixed model. There was no difference between Groups C and G on the number of displayed follicles (16.85 ± 1.32 vs. 16.12 ± 1.48); number of aspired oocytes (13.80 ± 1.27 vs. 13.05 ± 1.25); recovery rate (82 ± 1% vs. 80 ± 2%); cleavage rate (76.32 ± 1.58% vs. 76.78 ± 2.11%); number of embryos (10.79 ± 0.94 vs. 10.08 ± 1.01); number of blastocysts (5.89 ± 0.66 vs. 4.48 ± 0.6); embryos number of Grade I and II (5.49 ± 0.64 vs. 4.17 ± 0.57); plasma concentrations of total cholesterol (85.76 ± 1.20 vs. 87.11 ± 1.18 mg / dl), triglycerides (14.98 ± 0.29 vs. 14.26 ± 0.27 mg / dL); HDL (35.12 ± 1.10 vs. 35.40 ± 1.0 mg / dL) and LDL (47.92 ± 1.58 vs. 48.85 ± 1.51 mg / dL), for the respective groups. It was concluded that sunflower seed supplementation in oocyte donors did not increase the number and quality of oocytes, cleavage rates and the number and quality of blastocysts produced in vitro.

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