Iron is a trace mineral important to many metabolic processes such as oxygen transport, maintenance of the immune system, electron transfer and DNA and RNA synthesis. The aim of this research was to supplement inorganic iron during the offseason, and evaluate the effects on the morphology of hypopharyngeal glands of 6-day-old honey bees via morphological analysis of the acini. Twelve nucleus colonies were distributed in four groups: T0Fe, sugar syrup with no supplementation of inorganic iron; T25Fe, sugar syrup with supplementation of 25 mg L-1; T50Fe, with 50 mg L-1 and T100Fe, with 100 mg L-1. Ten six-day-old bees were collected every month from each treatment and its heads were embedded in metachrylate resin, which were cut using a rotating microtome to conduct the morphological analysis of the hypopharyngeal glands. It can be seen that the morphology of hypopharyngeal glands of 6-day-old honey bees supplemented with different levels of inorganic iron during the offseason did not significantly change (p<0.05). It is possible that the ferrous state of iron in the syrup was oxidized to the ferric state, an insoluble form of free iron, making its absorption more difficult. In conclusion, the supplementation of inorganic iron during the offseason did not significantly change the development of hypopharyngeal glands of 6-day-old nurse bees.