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A sensitive analytical procedure for the detection ofN-nitrosamides via their alkylating activity

Authors
Journal
Food and Chemical Toxicology
0278-6915
Publisher
Elsevier
Publication Date
Volume
27
Issue
7
Identifiers
DOI: 10.1016/0278-6915(89)90035-5

Abstract

Abstract Based on a method for trapping direct-alkylating intermediates with scavenger reagents possessing a nucleophilic side-chain and an N-nitroso group as a marker, a sensitive test system for the detection of N-nitrosamides and related compounds has been developed. N-Nitroso- N- tert-butylglycine is an effective scavenger reagent for this purpose. It reacts readily with diazoalkanes released from N-nitrosamides on treatment with alkali to form the corresponding esters which are analysed by gas-liquid chromatography using a thermal energy analyser. The method is easy to perform and specific for N-nitroso compounds that decompose to non-polar diazoalkanes on treatment with alkali. A determination limit of 2 ng solids/sample was established for methylated NTBG formed from N-methyl- N-nitro-sourea following treatment with alkali.

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