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Increased expression ofSKP2and phospho-MAPK/ERK1/2and decreased expression ofp27during tumor progression of cervical neoplasms

Gynecologic Oncology
Publication Date
DOI: 10.1016/j.ygyno.2006.09.015
  • Skp2
  • P27
  • Phospho-Mapk/Erk1/2
  • Cervical Neoplasm
  • Progression
  • Biology
  • Chemistry
  • Medicine


Abstract Objective. The objective of this study was to investigate whether the expression of SKP2, p27 and phospho- MAPK/ERK1/2 is associated with the progression of human cervical neoplasia. Methods. We performed immunohistochemical detection to stain formalin-fixed paraffin-embedded cervical tissues with anti-SKP2 and anti-p27 monoclonal antibodies and anti-phospho-p42/44 MAPK antibody. The study sample included 23 normal cervical epithelium, 25 low-grade squamous intraepithelial lesion (LSIL), 19 high-grade squamous intraepithelial lesion (HSIL), and 31 squamous cell carcinomas (SCC). In addition, 14 frozen cervical biopsies, including 1 normal, 6 HSIL, 2 adenocarcinoma and 5 SCC, and a human cervical cancer cell line (HeLa), were analyzed the expression levels of mRNA and protein of SKP2 and p27 by RT-PCR and Western blot analysis, respectively. Results. The expression of SKP2, p27 and phospho- MAPK/ERK1/2 were strongly associated with cervical neoplastic progression ( P < 0.0001, P = 0.006, P = 0.003, respectively; Fisher's Exact Test). In addition, SKP2 expression was positively correlated with phospho-MAPK/ERK1/2 expression (Spearman correlation coefficient = 0.480, P = 0.0002). The association between SKP2 and phospho-MAPK/ERK1/2 was significant after controlling for the four histologic grades ( P = 0.038, Mantel–Haenszel test). Conclusions. These results suggest that expression levels of SKP2, p27 and phospho-MAPK/ERK1/2 may serve as markers for progression in human cervical carcinoma and may also play roles in cervical carcinoma progression and cervical carcinogenesis.

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