Affordable Access

Publisher Website

Impact of myelin-specific antigen presenting B cells on T cell activation in multiple sclerosis

Authors
Journal
Clinical Immunology
1521-6616
Publisher
Elsevier
Publication Date
Volume
128
Issue
3
Identifiers
DOI: 10.1016/j.clim.2008.05.002
Keywords
  • Autoimmunity
  • B Cells
  • Multiple Sclerosis
  • Antigen Presentation

Abstract

Abstract The role of B cells in the pathogenesis of Multiple Sclerosis (MS) is incompletely understood. Here we define a possible role for B cells as myelin-specific antigen presenting cells (B-APCs) in MS. Peripheral blood B cells (PBBC) isolated from both MS patients and healthy controls (HC) were activated in vitro with either CD40L/IL-4 or a Class B CpG oligodeoxynucleotide (CpG ODN)/IL-2. Both activation techniques induced PBBCs to upregulate CD80 and HLA-DR, rendering them more efficient APCs than resting B cells. Although the CD40L/IL-4 B-APCs were highly effective in eliciting CNS-antigen specific proliferation by autologous T cells, CpG ODN/IL-2 stimulated B cells were not. Furthermore, CD40L/IL-4 B-APC induced responses by autologous CD4 + T cells were susceptible to blocking with anti-HLA-DR antibody, suggesting that T cell responses were specific for antigen presentation by B-APC. CNS-antigen specific CD8 + T cell proliferation was also blocked by HLA-DR, suggesting that CD8 + proliferation is in part dependent on CD4 + help.

There are no comments yet on this publication. Be the first to share your thoughts.