Affordable Access

Publisher Website

Pathogen associated molecular pattern-induced TNF, IL-1β, IL-6 and CXCL-8 production from feline whole blood culture

Authors
Journal
Research in Veterinary Science
0034-5288
Publisher
Elsevier
Publication Date
Volume
90
Issue
1
Identifiers
DOI: 10.1016/j.rvsc.2010.04.015
Keywords
  • Cat
  • Cytokines
  • Inflammation
  • Infection

Abstract

Abstract Whole blood culture ( C wb) is a method to evaluate leukocyte response to stimuli. We used C wb to evaluate the inflammatory response to pathogen associated molecular patterns (PAMPs) in cats. Blood was collected from diluted with RPMI and stimulated with various concentrations of lipopolysaccharide (LPS), lipoteichoic acid (LTA), peptidoglycan (PG) or control (PBS). Multiple concentrations of LPS, LTA and PG significantly stimulated tumor necrosis factor (TNF), interleukin (IL)-1β and CXC chemokine ligand (CXCL)-8 in feline C wb. All PAMPs failed to stimulate IL-6 production and PG failed to stimulate CXCL-8 production. Lipopolysaccharide was a more potent inducer of IL-1β and CXCL-8 than LTA or PG and LTA is a more potent inducer of CXCL-8 than PG. Based on these data, PAMPs from gram positive and negative bacteria induce TNF, IL-1β and CXCL-8 production in feline whole blood. Cats appear to be relatively more sensitive to gram negative compared to gram positive bacteria.

There are no comments yet on this publication. Be the first to share your thoughts.