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Characterization of dextrans by size-exclusion chromatography on unmodified silica gel columns, with light-scattering detection, and capillary electrophoresis with laser-induced fluorescence detection

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
790
Identifiers
DOI: 10.1016/s0021-9673(97)00731-0
Keywords
  • Dextrans
  • Polymers
  • Polysaccharides
  • Aminonaphthalene Trisulfonate

Abstract

Abstract Unmodified silica gel size-exclusion columns were used in an on-line combination with light-scattering detection for a size characterization of dextrans. The influence of electrostatic interactions on analyte retention was briefly investigated. Size-exclusion chromatography was also used for evaluation of the fluorescence labeling procedure for dextrans with 8-aminonaphthalene-1,3,6-trisulfonic acid. The derivatives obtained through this procedure were used for electrophoretic measurements with laser-induced fluorescence detection. A comparison between the size-exclusion data and capillary electrophoresis indicates that the effectiveness of fluorescent labeling decreases with molecular mass of the dextran analytes.

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