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THE ROLE OF Ca IONS IN RESTORATION OF THE STRUCTURE OF INTERPHASE AND MITOTIC CHROMOSOMES IN PK LIVING CELLS AFTER HYPOTONIC STRESS

Authors
Journal
Cell Biology International
1065-6995
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
22
Identifiers
DOI: 10.1006/cbir.1998.0291
Keywords
  • Chromosome Condensation
  • Intracellular Calcium
  • Hypotonic Stress
  • Glutaraldehyde

Abstract

Abstract The dynamics of mitotic chromosome and interphase chromatin recondensation in living PK cells during their adaptation to hypotonic medium was studied. The recondensation process was found to be slowed down by the modification of plasma membrane with low concentrations of glutaraldehyde, while osmotic reactions of glutaraldehyde-treated cells remain unchanged. The effect of glutaraldehyde can be rapidly reversed by the addition of Ca 2+-ionophore A23187. Intracellular Ca 2+measurements show that the adaptation to hypotonic shock is accompanied by restoration of free Ca concentration, whereas the delay of chromatin condensation in glutaraldehyde-treated cells is paralleled by the decrease of Ca level. The mechanisms implying the role of low concentration of Ca 2+in chromatin compactization in vivoare discussed.

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