Affordable Access

Publisher Website

Dramatic improvement of crystal quality for low-­temperature-grown rabbit muscle aldolase

Authors
Journal
Acta Crystallographica Section F Structural Biology and Crystallization Communications
1744-3091
Publisher
International Union of Crystallography
Publication Date
Volume
66
Issue
5
Identifiers
DOI: 10.1107/s1744309110011875
Keywords
  • Crystallization Communications

Abstract

Rabbit muscle aldolase (RMA) was crystallized in complex with the low-complexity domain (LC4) of sorting nexin 9. Monoclinic crystals were obtained at room temperature that displayed large mosaicity and poor X-ray diffraction. However, orthorhombic RMA–LC4 crystals grown at 277 K under similar conditions exhibited low mosaicity, allowing data collection to 2.2 Å Bragg spacing and structure determination.

There are no comments yet on this publication. Be the first to share your thoughts.