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Re-assessment of phenotypic identifications ofBacteroides putredinistoAlistipesspecies using molecular methods

Authors
Journal
Anaerobe
1075-9964
Publisher
Elsevier
Publication Date
Volume
17
Issue
3
Identifiers
DOI: 10.1016/j.anaerobe.2011.04.002
Keywords
  • Anaerobe
  • Identification
  • Bacteroides
  • Alistipes

Abstract

Abstract Alistipes (previously Bacteroides) are strictly anaerobic gram-negative rods that resemble the Bacteroides fragilis group in that most species are bile-resistant and indole-positive; however, they are only weakly saccharolytic and most species produce light brown pigment only on laked rabbit blood agar. In this retrospective study, we re-identified 18 organisms previously identified phenotypically as “ Bacteroides putredinis-like”, but that did not produce pigment on routine media. The strains were identified with 16S rDNA sequencing and pigment production was evaluated on several different culture media. Only 12/18 strains had molecular identifications of Alistipes species, while the remaining strains phylogenetically resembled Butyricimonas and Odoribacter spp. Pigment production was not a reliable test for those Alistipes strains that are described as pigment producers.

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